Define one hundred as the largest value in each data set, the value in the last row in each data set, a value you enter, or the sum of all values in the column. Define zero as the smallest value in each data set, the value in the first row in each data set, or to a value you enter. To normalize between 0 and 100%, you must define these baselines. ![]() Click OK which will bring up the Parameters: Normalize dialog. Then select Normalize from the "Transform, Normalize." section of the analyses at the top of the list. To normalize, click the Analyze button in the Analysis section of the toolbar. If you've defined the top and bottom of the curves by normalizing, you shouldn't ask Prism to fit those parameters. If you then fit a sigmoidal dose-response curve to the normalized data, be sure to set the top and bottom plateaus to constant values. ![]() One example of where normalizing can be useful: Investigators who analyze dose-response curves commonly normalize the data so all curves begin at 0% and plateau at 100%. If you can't get Normalize to do what you want, take a look at the Remove Baseline analysis which can do some kinds of normalizing. ![]() Normalize the data to convert Y values from different data sets to a common scale.
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